FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1475323
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTT6500.00725137868.9683751
CTCTCAT8150.0050201258.0467791
CTTCGAA11001.3737145E-47.94924161
CTCACGG8800.0099791137.4524141
CTTCAAG20204.53947E-87.21465831
CTCTGAT10100.00419819637.21465831
TCTTACT10350.00583019256.97501043
CTTTGAT25501.4551915E-96.8581691
CAAACGA10650.00702480046.842071
CCAATCT11850.0028925086.7013083
CTCAAAA19952.0539028E-66.5745611
CTTCCAT27155.6224962E-96.4413751
CAACAGG13800.00183949536.3363531
CTCAAAG13900.0019952026.29076671
CTTCGGT13350.00908281756.00410941
CTTGGAA15050.00485088075.8100771
CAAAAAG33251.1714292E-85.6979531
CGATTTT21605.6002034E-55.68172744
CAACAAC20751.6805044E-45.61874151
GACAACA15700.0085827045.5178112
Produced by FastQC (version 0.11.5)