FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933950
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTAT9402.1830601E-610.0053191
CTCCCAG8508.006769E-59.3624441
GTTCTAT8350.00665206977.79778961
CTCCATT9800.00335273337.38226031
CTTTAAT11300.00171225537.04254631
GCAAATC10300.0056251737.0002052
CAAATCA12000.00334040266.60935973
CTTTAAA12450.0047380856.392031
AAAACTT14300.0030841416.05052663
AAACTTT14900.00487540345.80688144
CTTTTAT19700.0033070775.1413511
TTTAAAA22606.5587676E-45.10457372
Produced by FastQC (version 0.11.5)