FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662045
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAGA11402.7901115E-79.4656271
CCCACGA8004.7754656E-48.9923461
TCGATTC8055.2689895E-48.9042033
CTCCCGG15754.9296432E-88.2215731
CCCAAAG12055.407222E-57.7610281
CTCAGAA10407.535976E-47.6089071
CTTCAAT26551.0913936E-117.31580641
CTCCAAC12054.5608298E-47.1640261
CTTTCAA31350.07.1135621
CTCAATT11150.00157990667.09709741
CCCACGG10200.0052358137.05281971
CTTCAAG22558.141797E-97.01841551
CTCCCAT12150.00390017946.5129741
CTCGAAA12250.00424901956.4598071
TTATCCC13450.00165810036.3951388
CATTGAG15007.938041E-46.23469261
CCTCTGT12950.0075737716.11062861
CAACCAA13000.0078820726.0871261
ATTGGTT15450.00113328216.05309961
CTCTTGT13100.0085323096.04065941
Produced by FastQC (version 0.11.5)