FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886789
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2755.856189E-620.80787
CACGTCT2857.972065E-620.0788359
ACACGTC3406.9371617E-414.7269388
GCACACG5752.4509127E-411.1955016
AGAGCAC6506.220699E-511.0053663
GAGCACA6053.9202048E-410.6415524
AGCACAC5500.002125690210.40395
CTTATCG8450.0078248497.65753171
TCAATGC12500.00555517766.2950693
Produced by FastQC (version 0.11.5)