FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1897200
Sequences flagged as poor quality0
Sequence length100-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAACT7151.4908267E-410.097511
CACACAT7251.7151557E-49.9582341
TTTAAGG10900.00126592957.2471687
CCTCGAA16607.4044838E-66.9587661
CCCAGTT13802.7591173E-46.8011851
CTTCTAC13903.013751E-46.75225541
TCGAGGG13500.00169327096.38336377
CTTCAAT25104.2451575E-76.04040241
CTTCTAT15700.00131652985.97811171
TACAGAT14450.0036223775.9647963
CCTTGGT14550.00369344955.95440771
CGAAGGG16300.0020612775.75805851
CCCGATT15200.0060018155.69977861
CCCAGAA16850.0030575435.570111
CAACGAG18150.00121344295.56892971
CTTTGGA18400.00144275495.4932651
CTTCAAA32002.201723E-75.4147891
CTTCAAC25652.3708993E-55.34794041
CTTCGAC20509.76986E-45.28272151
CTTCCAG35757.3534466E-85.25070521
Produced by FastQC (version 0.11.5)