FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954759
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAAT3150.00595334813.993181
CCATTAC7250.00161555379.1196931
CCCACAT8154.65724E-49.0140121
ATGTTCA10408.062524E-58.3207894
AACAATT11101.7164256E-47.79728032
CTCCTTT20204.834874E-97.6373671
CGTTGAT10150.004065667.2378511
CTCATAT10350.00491610647.09798961
CCCGGAT13802.225565E-46.920541
CTTAAAA12807.144896E-46.8872681
CTTCAGA10700.006787856.8658131
ATGATAC12708.0726E-46.81387475
GAAGAAC18806.704553E-66.52192742
CTTTGAT17257.368623E-56.3881911
AGAGGAT14050.00205183566.2745221
CATCAAT17100.00295732385.5849971
CAAAAGA20105.9283024E-45.48240231
CTTTTCT22901.0389224E-45.4536741
TCCTTTT25851.5423381E-45.0222332
Produced by FastQC (version 0.11.5)