FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1505620
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGAG4200.003000163512.127241
CTCAGGA10004.4745993E-69.4592471
CTTAAGT6950.00119991979.4226051
CTTCGGA12851.3747012E-79.06003951
CTTAAAC7400.00212523258.8496071
CTCAAGA13252.1984488E-78.7865291
CTTGTTT18359.822543E-118.7236821
CTTAATT11803.8729195E-68.6329511
GTCCCTT7600.0027068658.6167231
CTAAGAA10205.7921767E-58.5604061
GTCCTTA7950.00406613348.23737051
CCCAGTT9753.3368068E-48.2092091
CTACAAT13302.1811375E-68.2064031
CTTCTAT18651.2350938E-98.1932021
CTCTGTT14357.40165E-78.1129971
CTACTAT9100.00153202397.99598261
CTTATTT19202.1791493E-97.9585021
CTTCGAA8250.0056744517.937831
CTCCAAG11251.8125675E-47.76143361
CTCCACT8450.00703403177.74995231
Produced by FastQC (version 0.11.5)