FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1418155
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAACT6707.8396464E-510.758741
CCCCGCT8000.00468173348.1094011
GGTAGAT8050.0049516838.05903151
ATCCAAT11000.00133952467.20835641
GTTCAAC11100.00155602657.10714865
CAAAGAT20053.6725032E-76.8308611
TTAAGGA11700.00271022486.7429177
CTTTGAT22002.6238558E-76.5530511
GTTTGTT12400.0047231376.39450931
CAACAAA19451.1481088E-56.3003631
TGATTTA15008.235892E-46.21552474
CAAAGTT14000.00244186526.17859131
AGATTTA14250.00315161476.0393764
CTCAAAG14450.0034788675.9861781
GATTTAA13250.0099044895.9539135
CACCTTT15850.00159699665.88223
CTTCTCC23907.4388536E-65.7304921
CAACAAG17809.6629065E-45.6694931
GTTTCAT17900.00103756535.63782021
TATTGCC15300.00694047475.62510737