FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651574
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC1800.004391513320.070172
TACGAGT1900.00601108319.0094689
CTCCGAT4409.65867E-716.4766961
CGAAACA4652.9651022E-514.0317671
CCTATAC3200.007210359413.5932741
CTCAAAT4907.230105E-411.8363191
GTCAATT4650.006751145210.8767372
CTTCTCT7300.00193854118.9380431
CTTTGAA10054.7970764E-47.93504521
AATTTTG10800.00107392347.3584975
CTTTTGC15201.5179256E-57.1543541
CTTTTTC26202.8903742E-96.64098831
CTCCTTT12100.0034370126.59067771
Produced by FastQC (version 0.11.5)