FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531398
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2254.816502E-827.4364597
CACGTCT2851.7796992E-824.0670669
ACACGTC2601.9524123E-723.7430888
GCACACG3951.0701875E-515.6283626
AGAGCAC4852.7729766E-715.555293
AGCACAC4451.9363542E-615.412295
GAGCACA4804.2508273E-614.2884784
ATTGTGG4554.066921E-513.5674796
GTTTCGG4200.00465824111.4318576
GAAGAGC7101.8969213E-510.6709821
AAGAGCA8852.2139143E-48.5246512
AGTCACA2706.1723986E-67.62856120-24
ACAGTCG3350.0051846656.8010945145
TCGCTTA3052.5144895E-46.29760330-34
CAGTCAC2700.00280639836.10284920-24
CCAGTCA2700.00280639836.10284920-24
CTCGTAT3153.7867855E-46.100894535-39
GTCGCTT3400.00101229045.6493230-34
CAGTCGC3858.299798E-45.347784525-29
AGTCGCT3400.0061689115.248168525-29
Produced by FastQC (version 0.11.5)