FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449596
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGC2050.009386037517.5944548
CTCGATT3153.5245332E-416.0967521
CCTCCGT3558.7348867E-414.2830341
CTCAAAG4350.00402729311.6562681
CCTCAAA4450.004771904611.394331
CTTAATT4600.006108639811.0227761
CTTCTAT5500.001910642610.5360561
CTTGATT5550.00206004210.4411361
CTTTTAT9904.146279E-69.5117171
CTCCTTC6600.0086096358.7800461
CTTTCTT11352.1057247E-47.65836671
TTTTGCC10608.929507E-47.4859436
CAAAAAG12300.00410993546.47796151
Produced by FastQC (version 0.11.5)