FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764964
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCAAT4851.5858313E-716.3415761
CTACGAA2950.00438105314.6545421
CCCACAG5200.001252851911.0848461
CACAAAT8003.652276E-610.8077241
CACATGG6709.378512E-49.6785591
GATAACA7050.00153528839.1703912
TCGATCT6300.0063024319.1218713
CTTTTAT15102.3359462E-88.5889211
CTTCAAG9753.7032872E-48.1288871
GATTTGG8500.0083380367.6030475
ACAAATT12357.455139E-57.561552
CTTTGAT12558.764544E-57.4635021
ATGTTCA9750.0034296267.36477475
AGAAATT15853.9934293E-67.2514554
CTTTTGA15151.5821E-57.13381151
CTTTATT13800.00208441876.26534751
CTTTCCA15259.4857643E-46.1420951
CTTTTGC16754.4588168E-46.0222151
CATAAAT13250.0094274295.98163371
CTTCTTT24506.734761E-55.2935791
Produced by FastQC (version 0.11.5)