FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832144
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2900.031.6764077
ACACGTC4352.6993803E-919.4931748
AGCACAC4656.177288E-918.2355485
CACGTCT3653.8115068E-617.423699
GCACACG4401.2632481E-616.0597156
GAGCACA4402.2438875E-514.4537454
AGAGCAC4402.2438875E-514.4537453
GAAGAGC9700.00366876137.31426671
TTCACTT13900.00281260776.10038149
GAAAGAT18100.0086826075.095741
Produced by FastQC (version 0.11.5)