FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580566
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC14004.7925823E-78.3487991
CTCAAAC11753.2587042E-58.0823471
CTCCAGT11602.4674396E-47.55710171
CAACGAT14857.832504E-56.8870561
CTTCGAG12300.0037759686.5331051
CCCAGTT12400.0041095816.4804181
GGATTAC12700.00592514876.25688036
CAAATGA15509.793756E-46.12694031
AATTTCC20803.1196963E-55.90411045
CCATAAT16300.00179065145.8262321
AAGACTT15250.00617424355.68506243
GTTGGAA15450.00630931325.6739411
CGCCAAG21202.2040377E-45.51335761
CTTCCGT18950.00180264465.39698031
CCCGAAA17700.00475565345.36541
CTTTCAG26302.953371E-55.277521
CAAGCAT18350.00725870955.1753451
CCTCCAT18550.0082377685.1195461
Produced by FastQC (version 0.11.5)