FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802865
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACAG5250.001171969111.1737971
CCCCCCC1603.5139412E-510.094918125-129
CTCAAGG7502.0603003E-49.7770731
CTTTAAA8801.0086062E-49.1660051
CCTCGAT7400.00197892928.9182741
CTTTTGG8356.039921E-48.7818011
GTTTTAA8000.0040086758.2494041
CTTTGAA12456.383182E-57.6567431
ATCAGAT8550.0086827827.5687323
ATATTGC10750.00107624137.35751156
TTATTGA9800.00355883187.33703578
CTTCAAG11500.00178652717.0139861
AGAACTT10700.0083509956.71990254
CTTTTCA15800.0011761766.033321
CAAAGAA16350.00177195695.8303641
CAAAAAG19850.00307027275.1717511