FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1923583
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCACT7101.3543072E-410.1939761
CTCGGGA7803.48894E-49.2791331
CTCCTAA7803.48894E-49.2791331
CTACCGA7954.2218654E-49.1040541
CACCCGT11603.1177005E-58.1112421
GTAGGAT8150.00533610867.99257851
CCTTAAC8250.0059536697.8956991
CTCGTAT10105.163813E-47.8826691
CTCAGAT8400.00699736227.7547041
CTCCAGG9400.00222226567.6997061
CTCACCG15051.3578954E-57.2136781
CCTCAAG13251.6247679E-47.1011631
CCCCAAT10350.0056883946.99296951
GTCTATT12456.182782E-46.97611951
CACAAGA13552.1392733E-46.9439411
CTTCAAT30657.2759576E-126.84809061
CTCCAAT14858.849184E-56.8234431
CTTAAAA22404.7224603E-86.78536561
ACAATTC17151.2262937E-56.72571473
CTCCGAT15201.1998993E-46.6663241
Produced by FastQC (version 0.11.5)