FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1425476
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT4201.1764792E-515.4768267
ACACGTC5150.001133670811.2194468
CACGTCT5350.001561529110.7973769
CCCGTCG4850.00805923910.6217741
CTCAGTT6505.851998E-410.18991
CTTAAGA8901.0140424E-59.9227491
CTTTAAA18851.3460522E-108.5891881
CTCTCAA8607.824009E-48.5574091
CCTTCGT8658.2878454E-48.5079451
ACAACCT9352.2999839E-48.4970813
CTCAAAT11602.5284186E-58.2475731
CTCTCTC8950.00116206058.2227621
CTCTGAT14507.294475E-78.1206871
GAGCACA9803.8187992E-48.1069094
CTCAGAA10104.3157532E-48.0151581
CTTACAT8350.0057099027.9322571
CCCACCG10355.61027E-47.8215551
CTCTTAA8500.00669859077.79227731
GCACACG10256.1821373E-47.75099566
CTCCAAT11401.8526062E-47.7467081
Produced by FastQC (version 0.11.5)