FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372032
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT5351.16874035E-412.1241917
CACGTCT6203.5692967E-511.6227549
CACAAGT6002.8326744E-411.0228711
CTCATTT9853.2534317E-69.6986351
CCCAAAG11151.5727674E-69.2269171
CAAACCT7200.00151364449.1857251
AGCACAC8004.68698E-49.0089485
CTCAAAG12507.7681034E-78.81829551
GAGCACA7500.0026175548.648594
ACACGTC7550.0027799558.5913148
AGAGCAC7800.00373200128.3159513
CTCCAAA12608.190664E-68.165091
CCCCAAG8450.00643739247.82689051
CTATTCC9450.00243925197.6266234
CTCAAAA15953.13264E-67.3716171
CAACCTT11100.00120179157.28237721
CTTAAAA13251.3462837E-47.2099281
CAATGAT19007.989347E-76.9618131
CAAATCC18204.097803E-66.73196133
CTTCTAT12600.0045645526.4154271
Produced by FastQC (version 0.11.5)