FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628597
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGGG2600.001824024416.7124461
CTCAAAG6204.383083E-410.5126671
CCCAAAA4850.00906951110.4524571
CTTGAAA11502.8608156E-79.4461651
ATCAGAT7600.00282043458.5761231
CTCAAAA8750.00108721628.2766391
CTTCAAC8000.00448207658.1473171
CCTTGAT8150.0052973417.9973661
CCCCCCC2455.275871E-47.07399295-99
CTTCTTT15350.00644685.6615451
Produced by FastQC (version 0.11.5)