FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5112868
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAGT22758.019924E-97.02509071
CCCACAC26201.9585059E-86.3773051
CTCAAGA32751.4333636E-95.9891221
CTCAAAA69050.05.8916241
GTAGATC16300.00215526885.7370592
AGTTAAG17759.6042495E-45.67304946
CTTCGAT52700.05.65176251
CTCCCCT20701.6999348E-45.6151361
CTCCCGT28556.9583257E-75.5979271
CTCAGAT24701.13980495E-55.58814051
CAACGAA56300.05.54843761
CCCCGAT24951.3495604E-55.5321471
CCCAATT29009.4002644E-75.51106261
CTCAAGG32302.3578468E-75.3978331
CTTAAAA56050.05.31396771
CACAGAT49004.3655746E-115.18898771
CCCAAAC31003.354775E-65.15551041
CGACACT25501.1183074E-45.12794071
CTTAAAT41109.960786E-95.12586161
Produced by FastQC (version 0.11.5)