FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences885069
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT4907.265962E-411.8302141
CCCATTA4650.006611877610.9079661
CCTACAT4900.00975120710.3514381
CTTACAT6150.0048139399.4256981
ACTTTGG8559.286884E-59.2359746
CTTGAAT10357.204279E-58.4011661
TAAAACC9000.00156685977.9769742
CTTCAAA17452.9192961E-87.88963371
CTCAAAA12356.698454E-57.6273741
GTCAGAT8700.009467477.49586771
CAACAAT11702.987374E-47.4318011
CTTCTAT12759.9448014E-57.3880841
TCAGATT11803.6645745E-47.30095962
CTTTTAC14004.0060033E-57.24600551
CTGTAAT11300.00168373237.05363371
CTTTAAA12808.3553285E-46.793131
CTTCAAT13953.0059286E-46.75254961
ATCAGTT13100.00108700496.63756281
CAGATTT14354.7488319E-46.50387433
CTTTGAT14504.815317E-46.4964191
Produced by FastQC (version 0.11.5)