FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1163732
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAGT4300.003543931811.8599621
CTTAATG6708.480008E-49.78636551
CTCTGAT8901.2254808E-49.0044351
CTTTAAG8104.762179E-48.99432851
CTCAAGA8558.1604044E-48.5209431
CCCAGGC7800.00338442418.4062381
TTTTAGG8200.0057753877.92196377
CCTCAAT10205.3442095E-47.85680961
CTTCCAT18751.1496013E-87.77109961
CTTAAAG9750.00297961367.4722111
CAACGAT11200.00144803877.1553091
CCTCGAT10450.00585495086.971681
GTCAAAT10450.00585495086.971681
CCCATTT11550.0020048336.93848131
CTTTTAT20504.4836816E-76.75232741
CGATCAT10800.0080534766.7457461
CTTTAAT15151.0535029E-46.73238751
GATTCTC10800.0088155066.6828515
CTCCGAT10950.00919963056.65333841
CCTCCAT13150.00106814416.6482791
Produced by FastQC (version 0.11.5)