FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881910
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTGA8205.2130476E-59.7366781
CTTCAGA7300.00191937868.9485291
TAAACTT8205.8361655E-48.8123553
CTCCTGT8356.688951E-48.6925171
CTCAACA7900.00392517638.2688941
CCTTCAT10701.0411146E-48.1400961
CCTTGAT10701.0411146E-48.1400961
CTCAAAA13451.8825689E-47.01541041
CTTCAAA14808.138646E-56.8659141
CTCTGCT10800.0083444056.7206041
TTGATTT22452.4595702E-66.11565733
CTTGAAA16954.7286646E-45.99501661
CATAAAA16400.00207679675.7534921
TTTGATT18000.00656273875.21946532
Produced by FastQC (version 0.11.5)