FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666143
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC2500.001547299317.128728
CACGTCT2952.3993928E-416.9313589
CCTGCTA2900.00364192615.0670481
CCTACCA3456.761418E-414.7758971
CCCGTCT3456.761418E-414.7758971
CATATGA4150.00272274712.2835781
CCTCTCC4957.5818424E-411.7695461
GTCCTTT8205.396942E-48.8809831
ACATGAT6550.0091432728.7169063
CTTCTGA8050.0045099498.141821
CTTCAAT12701.0244568E-58.0278491
CCTTGAA8250.0056236147.9444431
CATCAAA13452.2013333E-57.58019971
CCTGTTT11550.00201023886.9356251
TGTTTTC15300.00731020845.59762053
CACCAGT4754.7313122E-55.41492325-29
Produced by FastQC (version 0.11.5)