FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461337
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCT4050.002457608512.451191
CTCTGCT4806.4065796E-412.0065061
CCCAAAA4550.005866471711.0829281
TCTGATT6708.840863E-510.6285612
CTTCAAT7200.0018093769.0048791
CTATTAT6400.00699822239.0048791
CTTTGAT8054.994206E-48.9489481
TTTGATT9302.5191193E-48.4228492
TTCCAGT8250.0068668757.7685122
AATTTTG9650.0033576187.3794165
CTTTTTT21055.8948917E-66.16010761
Produced by FastQC (version 0.11.5)