FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418490
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT1950.00676777418.6211431
CCCAAAT3554.621286E-516.3656251
CTCATAT3706.5840955E-515.7021541
CTCTTAT3250.00789219913.4072241
CTCTCCT3250.00789219913.4072241
TCATATT8154.3425825E-610.6493852
CTTATAT5900.0033481419.8471141
CTTCCTC5950.00359022049.7643651
AATTTTC8400.0070253777.7484025
ATTAAAA11600.00216167986.88661341
CTTTCTT13400.0097353375.96154551
Produced by FastQC (version 0.11.5)