FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1828331
Sequences flagged as poor quality0
Sequence length100-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT6450.018.6917827
CACGTCT8301.4551915E-1114.5255419
ACACGTC8856.1118044E-1012.821488
AGCACAC10801.3935278E-810.5064925
GCACACG12451.2172677E-89.6836946
AGAGCAC11855.8289515E-89.579993
GAGCACA12401.1794327E-79.1513164
AAGAGCA21954.685353E-86.78812
CTTTTAA17501.3664737E-46.1088271
GAAGAGC26652.6488306E-75.88342331
AGATGGT16450.00284210835.60452656
CCAAATC15200.0072739915.60145852
AAGATTC15450.0087063485.510823
GTTCAAA17950.00748997145.16159061
CTCGAAA20850.00145396935.1273131
CTTTGAT22708.2345254E-45.02341131
Produced by FastQC (version 0.11.5)