FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465492
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT2800.002836314715.6419731
CTCCAAT3650.001016749413.99920751
CTCAAAT4402.7518702E-413.27197551
CCCGAAT3350.00932229913.0738861
CTCCATT5801.5738698E-512.5854951
CTTCTAC4800.00791479610.645231
CCCTGCC5800.002786170710.0683961
CTTGAAA8200.0052072228.0117421
CATGGGT2500.00473439136.3934593120-124