FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974535
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAAT2000.00790371318.129851
CCCAAAC4957.8593614E-411.7203071
CTCAGAT5100.001009355611.3755921
CTTAAGA4600.006064820611.0355611
CTTGGTT6355.4012646E-410.278341
CTCATAA10054.9692408E-69.3806181
CTCCAAT8701.0055228E-49.1691191
CTCATAT8801.1388511E-49.0649251
CGTTTAT8801.1388511E-49.0649251
CTTACAT6650.00908763158.7241381
CTTCGTT9300.00196018637.79778531
CTAAAAT13051.5607844E-57.7798591
CTCAAAA14105.247126E-67.714831
CTCAAAT9500.00241584967.63362071
CAGGTTT8600.008800027.5576963
CTTTAAA20609.078576E-97.3927541
CTTAAAA15703.010391E-67.3905121
CTTGGAA10800.00103311467.38623481
TTAATCC9850.00358549977.3317753
CCTTATT11050.00131741627.21912531
Produced by FastQC (version 0.11.5)