FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339293
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTA3300.00906372813.1323078
TTAGATT6450.00730330128.9604792
CTCCATT11402.1964835E-47.63340761
CTTCAAA25502.7284841E-117.39392851
CTTTAAT16951.2155615E-67.2731291
TCCACAG12000.00328135126.6211187
CTTTTGT22252.8871E-76.5184161
CTTAAAG12400.0044360686.43299341
CGCTCTT12500.0048238056.3815291
TTCATGT13700.00186691786.327933
CTTCGAT14200.00267616156.1282291
CACCAAG14250.00278359886.1067261
GTTTCAA16150.0017498625.8373121
CTTTGTT20051.09943154E-45.78692251
TTCAAAC16450.00227962575.70924142
CTTTTAT24409.527461E-65.64684441
CCAAAAT17150.00357288425.4969441
CTCAAAA17250.00382687385.46507741
TTTCATG17750.0056004835.29112
Produced by FastQC (version 0.11.5)