FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161237
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTT3300.00894866413.1575317
CCCAAAC6756.0105795E-611.8379061
ACAAGTC6250.0055471999.2652953
CCCTTAC6350.00613949639.15171051
CAACCAA9302.0385272E-48.5920291
CAACGGA9954.224981E-48.0307411
CCACCGC11101.5809957E-47.8531561
CTCAAGA9300.00192865397.81093451
CAATAAG10255.810626E-47.7956941
CTAAAGA8500.00752488767.6914741
CTCAAGC9650.00277167687.5276371
CTATAAT11850.00270834296.7431111
CTTCAAA20655.3514486E-76.68373971
CTTTTAA17401.35476785E-56.6796961
CACAAAG12200.00367525876.54966071
CTTTTAT21359.5165706E-76.46460061
CTTTAAA16202.614818E-46.2776771
TGATTGA13850.0020636836.2716354
CCTGTAT12750.0058219616.26712661
CTCATCT12950.00684338166.1703371
Produced by FastQC (version 0.11.5)