FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1702598
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAAG7000.00121981149.4058091
CTCAAAG16357.756171E-98.5013431
CTCAGAA9753.149317E-48.253531
CTCAAGA11101.4579103E-47.9087881
CCCAAAT10758.9688855E-47.485761
CCCAGAT14400.00283828636.0963581
CCCATAT15950.00135794645.9625821
ATTCCAG18252.017289E-45.9372468
ATTCAAT22801.687028E-55.77549651
CTTATTT27954.018675E-75.75827741
CTTTCAT33351.1477823E-85.7033391
CTTTGAT29852.402121E-75.6368331
CACAGGA17050.00300672025.57789951
CTCCAAT15800.0079540285.55617431
AGATGCC17200.00387328075.4597356
CCCATTT18950.00177081775.40468651
CTTTTAA33053.2684693E-75.31240841
CTTATAT22154.0307693E-45.28442761
Produced by FastQC (version 0.11.5)