FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775728
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTCAT4856.595733E-411.9669771
CTTCGAA5250.001281225111.0552071
CCCCGGA4600.006044043711.0401881
CACCAAT8507.760039E-59.3887981
GTTCATT9502.595169E-59.1641861
CTATGAT8356.71675E-48.68861
CTTGTAT8457.5612124E-48.5857751
TTCAATT10155.8446552E-58.5524082
CTCCGTT7650.00294664258.5352721
CTTTTAA11101.7358667E-58.4968231
CTTTAAT11251.8717835E-47.73864561
CTTCAAA14256.0528037E-67.6368211
ATCAAGG10250.00520796567.0556547
CTTCCTC13652.2677846E-46.9095051
GTCAAAG10500.00638043746.9095051
CTCAAAA10600.00699365936.84432131
CTTTTAT14103.3721846E-46.68898871
CTCTTTT18504.7554313E-66.66673851
TTGATTT19007.567063E-66.4707773
CTCCTGC12650.00542954546.30867861
Produced by FastQC (version 0.11.5)