FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12894117
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGCTACGTGTAGATCT12030719.330386873331458Illumina Single End PCR Primer 1 (96% over 32bp)
TATATATATATATATATATATATATATATATATATATATATATATATATA797740.6186852500252635No Hit
ATATATATATATATATATATATATATATATATATATATATATATATATAT489510.3796382489781968No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGCTACGTGTAGATCTC324970.25202966593214565Illumina Single End PCR Primer 1 (96% over 31bp)
NATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGCTACGTGTAGATCT168240.13047810873749632Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAGAGCGTCGTGTAGGGAAAGAGTGTGTGGCTACGTGTAGATCTC134770.1045205344421801Illumina Single End PCR Primer 1 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC3466700.055.848299
AGAGCGT3542900.054.8509678
AAGAGCG3623250.054.045057
CGGAAGA3790900.052.462324
GAAGAGC3759000.052.423966
TCGGAAG3834600.052.133593
ATCGGAA3908100.051.4793552
GATCGGA3949050.051.4445721
GGAAGAG4002350.049.5003785
ATCGGAG80250.033.8420372
TCGGAGA88500.028.1711353
GACGTCG34400.024.4358279
TCGGAAA67650.022.7281513
CGTATCA1883600.022.15571660-64
GTATCAT1907250.022.10398960-64
CGCCGTA1932600.021.80044755-59
TCGCCGT1945300.021.68619255-59
GCCGTAT1913350.021.65254655-59
TATCATT1945450.021.609460-64
ATCATTA1930100.021.51175760-64
Produced by FastQC (version 0.11.5)