FastQCFastQC Report
Tue 26 Sep 2023
EGAF00005014388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33163853
Sequences flagged as poor quality0
Sequence length20-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGCAACTGGTGTAGATCTC385740.11631338493751013Illumina Single End PCR Primer 1 (96% over 33bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGCAACTGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA339040.1022317883268871Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)