FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22960580
Sequences flagged as poor quality0
Sequence length20-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA36600.024.2431681
TATGCCG40450.023.8296058
CGTATGC44200.022.7635486
CTCGTAT44300.021.4415054
TCTCGTA45700.021.0925753
TCGTATG47450.020.1663785
ATGCCGT57650.017.4524089
TCGGAAG52400.017.3363762
ATCTCGT60450.016.4256552
CGGAAGA72300.012.9431083
AATCTCG93550.010.91500951
GTATGCC98300.010.8795647
CGATCTA36050.09.79450830-34
TATCGGA16404.5835203E-46.01151751
CACACGT106550.06.009069
ACACGTC60650.05.986005310-14
TGCCGTC65500.05.919776445-49
GCACACG104100.05.8124948
TCACACG72300.05.538945725-29
CACGAGC41158.1574696E-45.0275364145
Produced by FastQC (version 0.11.5)