FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24301765
Sequences flagged as poor quality0
Sequence length20-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTGAGGATCTCGTATGCCGTCTTCTGCTTGAAAAA404820.16658049322754953TruSeq Adapter, Index 11 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTGAGGATCTCGTATG325310.13386270503397593TruSeq Adapter, Index 11 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA155100.0107.1386951
TCGGAAG184700.089.664982
CGGAAGA201250.082.1853563
AGAGCAC334000.049.5827837
AGCACAC350600.047.2555359
GAGCACA365150.045.3913738
GAAGAGC383600.043.3364645
AAGAGCA466550.035.886896
GGAAGAG605900.027.8991344
TATGCCG153700.023.7732645-49
CTCGTAT150600.023.06493640-44
TCTCGTA151900.022.8209140-44
TCGTATG151750.022.30202140-44
CGTATGC156200.021.73937645-49
ATGCCGT167900.021.72039845-49
ACACGTC182950.019.88085410-14
ATCTCGT168900.019.70199240-44
TGCCGTC186850.019.16821345-49
CGTCTGA186800.018.30637215-19
CGTCTTC207100.017.78935450-54
Produced by FastQC (version 0.11.5)