FastQCFastQC Report
Sun 2 Apr 2023
EGAF00005014392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24301765
Sequences flagged as poor quality0
Sequence length20-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTTGCGGTGTAGATCTC345100.14200614646713933Illumina Single End PCR Primer 1 (96% over 32bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTTGCGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA322710.13279282389571292Illumina Single End PCR Primer 1 (96% over 32bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTTGCGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA284000.11686393971795876Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)