FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005014433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71839176
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC3420340.4761106948108647TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA834450.073.648691
ATCGGAA877200.070.1805952
TCGGAAG869200.070.1507343
CGGAAGA893050.067.8194664
GATCGGG83850.049.325951
AGAGCAC1276900.046.981798
GAGCACA1335600.044.824659
GAAGAGC1370800.044.1390276
ATCGGGA96350.042.08942
AAGAGCA1777250.034.199617
GGAAGAG1889600.032.50345
TCGGGAA135900.029.4493373
TATGCCG563600.024.30405445-49
CTCGTAT581200.023.57772340-44
TGCCGTC590600.023.05302445-49
TCTCGTA554700.022.92926640-44
CGATCAG599450.022.9056330-34
ATGCCGT599650.022.87436545-49
TCGTATG616000.022.31164240-44
ACGATCA646700.021.43833730-34
Produced by FastQC (version 0.11.5)