FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005014447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65516135
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGGTGATCTCGTAT788090.12028945236162054TruSeq Adapter, Index 3 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA344250.041.0566331
ATCGGAA370300.038.140152
TCGGAAG381400.036.9921233
CGGAAGA403500.034.8940354
AGAGCAC772400.018.5835488
TATGCCG172000.018.24385645-49
GAAGAGC850200.017.138816
GAGCACA841900.017.0580629
CGTATGC193400.016.15016745-49
CTCGTAT189600.016.01468840-44
CTTACGG208200.015.17484630-34
TCTCGTA189800.015.13451340-44
GCCGTCT216350.014.33509950-54
TTACGGT211550.014.30398930-34
ATGCCGT212000.014.29546445-49
ACTTACG229950.013.69538330-34
TCGTATG213650.013.45205345-49
TGCCGTC210200.013.42322850-54
ATCTCGT222700.012.8140140-44
CGGTGAT225300.012.65219935-39
Produced by FastQC (version 0.11.5)