FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005014480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63044613
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTACAGGTCGTGTAGATCT1148620.1821916172282634Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC612950.031.0422559
AGAGCGT662300.028.7619788
GATCGGA700900.028.6014941
ATCGGAA736500.027.1789952
TCGGAAG739100.026.8418943
CGGAAGA765550.025.7440514
AAGAGCG748200.025.6922687
CGCCGTA236150.020.04938955-59
TCGCCGT257550.018.24842355-59
GCCGTAT264650.017.99432655-59
GAAGAGC1179450.016.7835336
GTCGCCG280550.016.59224755-59
CGTATCA291250.015.94755660-64
TGGTCGC302250.015.88077650-54
GTGGTCG317350.015.27128250-54
AGGTCGT347550.013.28044135-39
TCTCGGT382200.013.08206845-49
GGTCGTG370350.012.85415235-39
CCGTATC287650.012.80687560-64
GGAAGAG1647300.012.3687235
Produced by FastQC (version 0.11.5)