FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49000189
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC308550.024.5079
GATCGGA319050.024.0452631
AGCGTCG264800.022.724359
AGAGCGT351600.021.6713148
CGCCGTA133850.020.55063855-59
ATCGGAA378400.020.4655882
TCGGAAG383850.020.076873
TCGCCGT143600.019.1250255-59
AAGAGCG406450.018.9965117
CGGAAGA411000.018.6624094
GTCGCCG151250.017.89885755-59
GCCGTAT159750.017.30956655-59
TGGTCGC175350.015.9589350-54
GTGGTCG184950.015.24816350-54
CGTATCA183400.014.90291660-64
TCTCGGT225400.012.77527945-49
CTCGGTG238850.012.00124945-49
CGGTGGT237600.011.77777250-54
GAAGAGC741850.010.8768666
CGTCGTG248000.010.83378110-14
Produced by FastQC (version 0.11.5)