FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005014533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72735160
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA215350.026.2588581
ATCGGAA257150.022.3663352
TCGGAAG275750.020.8235513
CGGAAGA308550.018.5849534
TATGCCG131550.012.48807145-49
CTCGTAT149250.011.33707240-44
TCTCGTA145850.010.53765140-44
CGTATGC155250.09.90923445-49
ATGCCGT174400.09.86871345-49
GCCGTCT181500.08.42009950-54
AGAGCAC731450.08.2560228
ATCTCGT187350.08.00997340-44
GAGCACA803250.07.57219369
GAAGAGC820500.07.5632026
CGTCTTC230300.07.347340650-54
CCGTCTT227800.07.07153450-54
TCGTATG174750.06.87849945-49
ACACGTC237950.06.80625510-14
TGCCGTC174900.06.797939350-54
CGTCTGA230150.06.6274415-19
Produced by FastQC (version 0.11.5)