FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005014547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73253715
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTCTGGATCTCGTAT1272160.17366491242116525TruSeq Adapter, Index 16 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA482050.049.726191
ATCGGAA520000.046.206852
TCGGAAG537850.044.5068173
CGGAAGA573100.041.6048664
AGAGCAC968000.024.7591558
GAGCACA1041750.022.9576349
GAAGAGC1069100.022.6286
TATGCCG274500.019.69172345-49
CGTATGC299300.017.91957745-49
TCTCGTA283300.017.65824940-44
CTCGTAT299300.017.58632340-44
AAGAGCA1448700.016.8989967
ATGCCGT315900.016.53271745-49
GCCGTCT328050.016.10148650-54
ATCTCGT321800.015.42396340-44
TGCCGTC315300.014.98674450-54
GGAAGAG1660750.014.9246665
TCGTATG324150.014.70292845-49
CGTCTTC369250.014.54445350-54
CCGTCTT374100.014.29387950-54
Produced by FastQC (version 0.11.5)