FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005014548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73253715
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGCAGCTAGTGTAGATCT1408850.19232471691026182Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC513650.045.808279
GATCGGA546800.043.5099561
AGAGCGT565250.041.7641038
ATCGGAA591250.040.368152
TCGGAAG609000.039.1592753
CGGAAGA647750.036.749134
AAGAGCG654400.036.353387
GAAGAGC1140900.021.3315756
CGCCGTA249000.021.11150455-59
TCGCCGT271700.019.31033755-59
GCCGTAT287400.018.33614755-59
GTCGCCG290500.018.04069555-59
TGGTCGC319000.016.6698550-54
CGTATCA317850.016.58536560-64
GTGGTCG333000.015.99948250-54
CCGTATC311650.014.9572960-64
GGAAGAG1718450.014.5106165
GGTCGCC328700.014.24650155-59
TCTCGGT396400.013.72601745-49
CTCGGTG424900.012.76441745-49
Produced by FastQC (version 0.11.5)