FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50071229
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTCTGGATCTCGTAT1771990.35389384989931044TruSeq Adapter, Index 16 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA517000.062.5018961
ATCGGAA556050.058.3290632
TCGGAAG563650.057.4011083
CGGAAGA587850.054.915364
AGAGCAC839750.038.2673958
GAGCACA877000.036.6089489
GAAGAGC910600.035.5685966
AAGAGCA1189550.027.3861897
GGAAGAG1295400.025.3234185
TATGCCG318100.023.06811545-49
CGTATGC335500.021.59083245-49
TCTCGTA320500.021.3528440-44
CTCGTAT334750.021.26682340-44
ATGCCGT343350.020.59890745-49
GCCGTCT347300.020.55284550-54
ATCTCGT346200.019.50806240-44
CGTCTTC376050.019.26684850-54
CCGTCTT382600.018.7702650-54
TGCCGTC347850.018.2194750-54
TCGTATG351750.018.12432345-49
Produced by FastQC (version 0.11.5)