FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45746919
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC190000.012.8588539
GATCGGA210250.011.9150271
AGAGCGT217850.011.4812058
CGCCGTA55150.010.936686555-59
ATCGGAA230600.010.8151772
TCGGAAG245350.010.3415833
CGGAAGA262550.09.6917044
AAGAGCG264450.09.5676327
TCGCCGT66950.09.11736855-59
GTCGCCG77100.07.935897455-59
GCCGTAT81550.07.556191455-59
TGGTCGC90900.06.826888650-54
GTGGTCG102500.06.209885650-54
CGTATCA102350.06.16219460-64
CCGTATC93150.05.572294760-64
GGTCGCC97850.05.3195155-59
Produced by FastQC (version 0.11.5)