FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42677947
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATTCGCTGTGTAGATCT521040.12208647243504941Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC291800.029.5090969
AGAGCGT325400.026.5288228
GATCGGA338000.025.4149321
ATCGGAA356850.024.3844052
TCGGAAG364250.023.9590743
AAGAGCG373700.023.1970837
CGGAAGA379100.023.0397014
CGCCGTA100250.018.8732155-59
TCGCCGT113650.016.75000655-59
GCCGTAT121650.015.708076555-59
GTCGCCG121650.015.50546855-59
TGGTCGC136900.013.85091650-54
GAAGAGC661550.013.4548466
GTGGTCG143750.013.26148350-54
CGTATCA144250.013.25731560-64
CCGTATC135250.012.77807960-64
GGTCGCC139550.012.24908555-59
TCTCGGT181050.010.89005845-49
ATTCGCT186600.010.27886930-34
CTCGGTG195550.010.03066545-49
Produced by FastQC (version 0.11.5)