FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44099714
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC401100.012.2665939
AGAGCGT435200.011.2887978
CGCCGTA98150.010.86866455-59
GATCGGA479650.010.2163241
ATCGGAA487400.010.1272862
TCGGAAG494000.09.945383
AAGAGCG500900.09.9093787
CGGAAGA510150.09.7867844
GCCGTAT117000.09.3158155-59
TCGCCGT116600.09.23589355-59
CGTATCA134050.08.36888860-64
GTCGCCG135200.08.13680355-59
CCGTATC134450.07.438440360-64
TGGTCGC149050.07.418710750-54
GTGGTCG158850.07.079626650-54
GAAGAGC784750.06.80523356
GGTCGCC158500.06.44686455-59
TCTCGGT199600.05.910953545-49
CGGTGGT216050.05.40650550-54
CTCGGTG226450.05.248500345-49
Produced by FastQC (version 0.11.5)