FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13173363
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC75350.020.3954059
GATCGGA79250.019.6936841
AGAGCGT82300.018.9373138
ATCGGAA85850.018.4104122
TCGGAAG90250.017.4299263
CGGAAGA99500.015.7367024
AAGAGCG100700.015.6212217
CGCCGTA29600.012.88221655-59
GCCGTAT33700.011.35796555-59
TCGCCGT34000.011.34303555-59
GTCGCCG39000.09.96315255-59
CGTATCA40200.09.77447760-64
TGGTCGC42700.09.06627250-54
GAAGAGC188250.08.8568146
GTGGTCG44550.08.75487450-54
AGGTCGT44450.08.54585335-39
CCGTATC38400.08.11815860-64
GGTCGTG48900.07.827461735-39
TCTCGGT57700.07.161361745-49
GGTCGCC45500.06.72353455-59
Produced by FastQC (version 0.11.5)